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The Biomek 4000 AMPure XP method allows users to select the number of samples to be purified, as well as user ID, sample, and reagent lot number tracking via LIMS data collection. Plasmid DNA fragments (1000 bp pGEM) produced by PCR were purified on Biomek 4000 using this method, producing equivalent yield and purity (260nm/230nm ratio) to amplicons purified usingthe manual process (Table 1 Identity Automation™ Plexor® HY System Setup for the Beckman Coulter Biomek® 4000 PDF (1024 KB) - English. Plexor® HY System for the Applied Biosystems 7500 and 7500 FAST Real-Time PCR Systems Technical Manual PDF (6287 KB) - English. Plexor® HY System for the Stratagene Mx3000P® and Mx3005P™ Quantitative PCR Systems Technical Manual PDF (11589 KB) - English. Plexor® HY System BIOMEK Water Engineering Equipment is the mechanical division of the True Water group. Width 300 - 4000 mm Bar spacing 12 - 100 mm Capacity 10 - 10 000 l/s Motored gears Sew or Nord Material SS304L, SS316L Multi Rake MR. Multi Rake MR is a modern mechanically raked bar coarse screen that separates debris and large screenings from water. pg 5 Directors: Q C Dickerson, S G Hall, D J Setter 5.C. ®Biomek 4000 Initial Deck Configuration (continued) Elution_Volume The Elution_Volume variable corresponds to the final volume of Elution Buffer added to each sample well (i.e., 40-100µl). If a volume outside of this range is entered, the default volume of 40µl or 100µl will be used. Note: EP059 · 7/15 ® ® ® ® ™ System Protocol for the Beckman Coulter Biomek® 4000 ® Promega 4 Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 EP062 · Revised 11/16 promega.com 5. ®Automated Processing Requirements for the Beckman Coulter Biomek 4000 Workstation Confirm that you have the instrument and labware requirements listed in Sections 5.A and 5.B for use of the Optimize dependability and walk-away time. The Biomek i5 is a recent addition to our liquid handling portfolio, representing the best of what has made Biomek an industry-leading brand - combined with enhancements suggested by customers around the globe. Ideal for medium- to high-throughput workflows 25 deck positions Biomek 4000 using this method, producing equivalent yield and purity (260nm/230nm ratio) to amplicons purified using the manual process (Table 1). In order to measure cross-contamination during automated purification, the 452bp standard from the KAPA SYBR Fast Universal qPCR Kit was used as template to generate a large volume of PCR fragments. These were then purified using the Biomek 4000 Automation with the Biomek 4000 platform can improve the throughput and reproducibility of conventional staining . of adherent cells. Applicability is demonstrated using mouse embryonic stem cells (mESCs) to detect the expression of developmental antigens in fixed cells following differentiation to cardiomyocytes which is relevant to stem cell biology research. The cardiomyocyte model Biomek 4000 Liquid Handling Automation Introduction This document describes the automation of Mo Bio PowerMag Soil DNA isolation kit (27100-4) for purification of microbial DNA from soil, compost, sediment etc. using the magnetic bead based sepa