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Analyzing Cell Death by Nuclear Staining with Hoechst 33342. Lisa C. Crowley,; Brooke Adapted from Imaging: A Laboratory Manual (ed. Yuste). CSHL Press Technical Data Sheet. Hoechst The BD Pharmingen™ Hoechst 33342 Solution is a reagent for the fluorescent staining of DNA and nuclei in live or fixed cells. Aug 24, 2018 - Example of cell staining with the Hoechst 33342 Staining. Solution. 1. which extend beyond the technical specifications of the products. Miltenyi Biotec. Description. Hoechst dyes are cell membrane permeable and stain DNA to emit intense blue fluorescence. They bind to DNA in the minor groove of poly-AT When bound to dsDNA, the maximum absorp- tion is 350 nm and the maximum emission is. 461. SPECIFICATIONS. • 1 mL at 200 ?g/mL. • Pale green liquid. • pH Optional: Remove PBS and mount cells in antifade-mounting media. 9. Image cells. Specifications. Visit bio-rad.com/PureBluHoechst33342 for more information. Hoechst 33342 binds preferentially to adenine-thymine (A-T) regions of DNA. Hoechst 33342 is used for specifically staining the nuclei of living or fixed cells or perform substantially in conformance with published Product specifications in Page: 1 of 5. Hoechst 33342 (hydrochloride). SAFETY DATA SHEET. 10/20/2014. Supersedes Revision: according to Regulation (EC) No. 1907/2006 asMar 12, 2018 -