enzyme for one-step RT-PCR of RNA targets up to 3 kb long. Tth DNA Polymerase (available from Roche Applied Science) will reversely transcribe.Expand Long Template PCR System is optimized to efficiently amplify large genomic DNA fragments around 20 kb long. Due to the inherent 3'-5' exonuclease (“ Some genetic and pharmacogenetic tests require the routine amplification of long DNA fragments from complex genomic targets. Assays targeting fragments >3 Amplification of a 17.5kb β-globin fragment from human genomic DNA using GoTaq® Long. PCR Master Mix. GoTaq® Hot Start DNA Polymerase alone (lane 1) or A protocol for amplification of long PCR targets. GoTaq® Long PCR Master Mix is a ready-to-use solution containing everything needed for long PDF (357k) long PCR protocols to amplify introns of a newly identified gene and nearly entire viral genomes. General method. Primer design. Successful primers will. PDF | Long-range PCR remains a flexible, fast, efficient and cost-effective choice for sequencing candidate genomic regions in a small number of.