Optimal Electroporation Condition for Small Interfering RNA Transfection into MDA-MB-468 Cell Line Rita Arabsolghar, Mozhgan Rasti Introduction Small interfering RNA (siRNA) transfection is a valuable tool for evaluation of expression of many proteins and analysis of many pathways in the cells, and medical application.1 In mammalian cells, upon transfection of gene-specific siRNA, specific Overexpression of PEA-15 in the MDA-MB-468 triple-negative breast cancer cell line inhibited growth and induced apoptosis. Correspondingly, in athymic nude mice bearing human MDA-MB-468 xenografts, tumor volumes were significantly smaller in mice treated intratumorally with Ad.PEA-15 adenovirus than in control mice. Combined, these results evaluate the viability of the MDA-MB-468 cells after treatment and activation with 3 EGFR ligands: EGF, BTC, or TGFA. EGF activity was measured as a function of receptor phosphorylation. Western blot analysis was conducted after MDA-MB-468 cell exposure to increasing concentrations of the three ligands. This was Maintaining healthy cells is the key to experimental success and reproducible research results. To give you confidence in the health of your cells every step of the way, we've highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No COSMIC - Cell Lines Project reported the following signals for MDA-MB-468. 132 Genes with gain 409 Genes with loss 57071 Genes with no signal Disease: Adenocarcinoma Origin: Established in 1977 from the pleural effusion of a 51-year-old black woman with metastatic adenocarcinoma of the breast; these cells carry a p53 point mutation at codon 273 leading to Arg-His substitution SOP: Thawing, Propagation and Cryopreservation of NCI-PBCF-HTB132 (MDA-MB-468) Page 4 of 25 Cell Growth Incubate cultures at 37°C; in a free gas exchange with atmospheric air A change regarding the extent of adhesion ? hereafter referred to as adhesion plasticity ? between adhesive and less-adhesive states of mammalian cells is important for their behavior. To investigate adhesion plasticity, we have selected a stable isogenic subpopulation of human MDA-MB-468 Mda Mb 468 Breast Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more Maintaining healthy cells is the key to experimental success and reproducible research results. To give you confidence in the health of your cells every step of the way, we've highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No Cytotoxicity of different extracts of arial parts of Ziziphus spina-christi on Hela and MDA-MB-468 tumor cells Abbas Jafarian , Behzad Zolfaghari , 1 and Kobra Shirani Department of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran Cytotoxicity of different extracts of arial parts of Ziziphus spina-christi on Hela and MDA-MB-468 tumor cells Abbas Jafarian , Behzad Zolfaghari , 1 and Kobra Shirani Department of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran ATCC mda mb 468 cells Mda Mb 468 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 165 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions