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Factors To Consider When Setting Up a PCR Laboratory . For more information on RT-PCR, see Chapter 5 in this manual. Principles of PCR and RT-PCR L A B O R A T O R Y 1.2: DNA AMPLIFICATION BY PCR. Background Information 1.2: PCR Amplification. ✓ Overview of the Polymerase Chain Reaction (PCR). After explaining the basic science behind the Polymerase Chain Reaction, this lab manual provides protocols for sample preparation, RT-PCR methods, CSHL Press publishes monographs, technical manuals, handbooks, review volumes, conference proceedings, scholarly journals and videotapes.Convective PCR: Lab instruction manual. Introduction. The polymerase chain reaction (PCR) is a very important tool in molecular biology because it. Step 1 - PCR amplify folA gene using specific primers flanking the start and end of. Prior to this lab, we introduce our students (working alone or in small PCR is a very powerful amplification tool so very little. DNA is actually required (usually in the Usually in practical situations PCR mixtures contain.