We use cookies to make interactions with our website easy and meaningful, to better understand the use of our services, and to tailor advertising. Context 1. product was used to perform a ligation reaction with pGEM ® -T easy ( fig. 21). The molar ratio of the PCR product to the vector used in the Promega Corporation. Загрузка Video describing the use of the pGEM-T Vector Systems. Promega E8051 Technical Manual. Glomax multi+ detection system. Hide thumbs. Page 4 Each detection mode has dedicated optics for the Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax The pGEM-T Easy Vector Systems are convenient systems to clone PCR products. They offer all of the advantages of the pGEM-T Vector Systems with the added convenience of recognition sites for EcoRI and NotI flanking the insertion site. Supplier Page from Promega for pGEM-T Easy Vector System II. Wizard® Genomic DNA Purification Kit (Promega) 500 isolations x 300 µl.. 0????. A1222. PureYield™ Plasmid Miniprep System (Promega) 250 preps.. pGEM®-T Easy Vector System I (Promega) 20 reactions.. pGEM-T Easy Vector System I. by Promega. Available SKUs: A1360 |. Login to view price. The pGEM®-T Easy Vector Systems offer all of the advantages of the pGEM®-T Vector Systems with the added convenience of recognition sites for BstZI, EcoRI and NotI flanking the insertion site. pGEM®-T Easy. Antibiotic Resistance. KEYWORDS pGEM-T Easy SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3015) AUTHORS Promega TITLE Direct Submission COMMENT Linearize at EcoRV to create a TA cloning vector. Iam using the pGEM T easy vector for cloning. Followed the ligation protocol as per promega instuctions. I tried to transform the ligated vector into We used the pcr product purified using SV gel wizard purifiying kit. This purified product was used for ligation with pGEM T easy vector. added 3ul of Производитель: Promega. Цена A1360 pGEM®-T Easy Vector System I. L2004 JM109 Comp.Cells, High Efficiency. Storage conditions. Promega. Alt Name: pGEM®-T Easy, pGEM-T Easy. Analyze The only difference between pGEM-T and pGEM-T Easy is in the multiple cloning site (MCS). The MCS of the pGEM-T Easy Vector contains sequences on either side of the insert that are recognized by the restriction enzymes Not I Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax Vector Features T-Overhangs for Easy PCR Cloning: The pGEM ® -T and pGEM ® -T Easy Vectors (a,b) are linearized vectors with a single 3?-terminal Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax Vector Features T-Overhangs for Easy PCR Cloning: The pGEM ® -T and pGEM ® -T Easy Vectors (a,b) are linearized vectors with a single 3?-terminal