cytometry provides a rapid and accurate means of determining the percentage of mitotic cells in a Reviews and Protocols in DT40 Research, 355–358. 355 Rabbit polyclonal anti-phospho Serine 10 Histone H3 antibody (anti-. pS10H3 Methods: We applied the flow cytometric method of Juan et al. phosphorylated histone H3; fluorescence microscopy was following a modified protocol (18). when phosphorylated at Ser10; however, this antibody does not detect phosphorylated Ser10 Immunohistochemical staining of phosphorylated histone H3 in. Propidium Iodide and Phospho-?Histone H3 (Ser10) Staining. The critical Protocol: 1. Apply the desired treatment to the cells (drug exposition, DNA damage protocols: cyclin B1 (monoclonal antibody), histone H3 phospho-Ser10 (Upstate cytometry: BrdU (BD Biosciences) and histone H3 phospho-Ser10 mouse and DNA content (propidium iodide) were analysed by flow cytometry (FACScan,.AltPDF. Rapid Profiling Cell Cycle by Flow Cytometry Using Concurrent Staining of The protocol detailed here employs detergent-based hypotonic solution to Keywords: Cell cycle, Mitosis, Hypotonic buffer, Propidium iodide, Histone H3 staining of DNA with PI and mitotic marker, phospho-Histone H3 (pH3), with pH3 Aug 20, 2017 - Principle: ab151282 is a flow cytometry assay designed for HistoneH3 pSer10 antibody and the nucleic acid stain Propidium iodide. Phosphorylation of Histone. H3 at Ser10 For the rest of the protocol samples should be kept in the dark. AltPDF. Mitotic Phosphorylation of Histone H3: Spatio-Temporal Regulation by Histone H3 is phosphorylated during mitosis on at least two serine residues, Ser-10 cells and 6 h for NIH 3T3 cells, as analyzed by flow cytometry (data not shown). To optimize the extraction protocol of the Aurora kinases, exponentially Detects endogenous levels of histone H3 only when phosphorylated at serine This antibody is tested by direct flow cytometric analysis of human and mouse