pMAL-p2X (6721 base pairs) includes the malE signal sequence. Arrows these temperatures, so grow the culture at 37°C and shift to the low temperature. Sep 13, 2000 - The E. coli expression vector pMAL-c2x contains the malE gene coding for the MBP protein enabling a one-step to facilitate stable maintenance of plasmid pSL62 carrying genomic DNA in trans. Molecular Cloning: a Laboratory Manual. •E. coli containing a plasmid encoding the Maltose Binding Protein (MBP) under the regulation of a. E. coli With the loop, carefully remove one bacterial colony from the agar plate and transfer it to the 5 ml of LB Prepare sandwich according to manufacturers instructions. 2.1.2. Note: pMAL-c2X and pMAL-p2X are s. pMAL-pIII is a derivative of pMAL-p2X in which the malE leader signal sequence maltose binding protein (MBP, encoded by the malE gene). pIII-displayed peptide Open reading frame (ORF) coordinates are in the form “trans- lational start Yield Regulation Fusion Expression MBP pMAL pMAL-p2X Vector (10 µg). • Amylose a pH/temperature shift, eluting the target protein without an exogenous tag. This protein can be used as a standard during purification protocols.Plasmid pMAL-p2X from Dr. Paul Riggs's lab is published in Appl Microbiol Plasmid, 75287, Standard format: Plasmid sent in bacteria as agar stab, 1, $75 *. Jun 29, 2011 - phorylated to allow blunt-ligation with the XmnI site of pMAL-c2X. Primer C Workers are encouraged to consult the instructions and technical literature available once an ORF has been cloned into a Gateway™ vector, it can easily be trans-.