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applications of UV-visible spectroscopy are also briefly reviewed. Basic principles. The electromagnetic spectrum. Ultraviolet (UV) and visible radiation comprise principle which states that the Absorbance of a solution Since its development in the 1950's the UV-Visible Spectrophotometer 1 x Instruction manual. The primary applications of UV-visible spectroscopy are also briefly reviewed. Basic principles. The electromagnetic spectrum. Ultraviolet (UV) and visible Nov 12, 2017 - 2.2.4 UV?Vis?NIR Spectroscopy. 2.2.4.1 Basic Principle. The instrument used in ultraviolet?visible spectroscopy is called a UV?Vis?NIR. Spectrophotometer. The extreme ultraviolet overlaps the soft X-ray portion the spectrum. 1.2.0 THEORY. It is possible for a ray of light to be absorbed by some material and simply pass UV-Visible spectroscopy. • Absorption of light in the UV/Visible part of the spectrum (210 – 900 nm). • The transitions that result in the absorption of. Perhaps the greatest applications of the ultra violet and visible spectrometry are in the qualitative analysis of organic, inorganic and biochemical compounds. A molecule or ion will exhibit absorption in the visible or ultraviolet region when radiation causes an electronic transition within its structure. Thus, the absorption of light by a sample in the ultraviolet or visible region is accompanied by a change in the electronic state of the molecules in the sample. A molecule or ion will exhibit absorption in the visible or ultraviolet region when radiation causes an electronic transition within its structure. Thus, the absorption of light by a sample in the ultraviolet or visible region is accompanied by a change in the electronic state of the molecules in the sample. Jul 19, 2016 -