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QIAprep Spin Miniprep Kit Protocol using a microcentrifuge. This protocol is designed for purification of up to 20 µg of high-copy plasmid DNA from. 1–5-ml selection guide at qiagen.com/products/plasmid/selectionguide . 27104. Reagents, Buffers, Collection. Tubes (2 ml). QIAprep Spin Miniprep Kit (250) QIAGEN Robotic Systems are not available in all countries, please inquire. Kit Contents. QIAprep® Spin Miniprep Kits. (50). (250). Catalog no. 27104. 27106. Sample to Insight__. Quick-Start Protocol. February 2015. QIAprep® Spin Miniprep Kit. The QIAprep Spin Miniprep Kit (cat. nos. 27104 and 27106) can be stored 27104 and 27106) can be stored at room temperature (15–25°C) for up to 12 months. For more information, please refer to the QIAprep Miniprep Handbook, December. 2006, which can be found at: qiagen.com/handbooks. For technical Prepare DNA from 10 colonies for each knockdown plasmid using a Qiagen Miniprep Kit (Qiagen 27104). 8. Screen Purified fragments are then cloned into the pGEM-T-Easy vector (Promega A1360) following the manufacturer's instructions. QIAprep Spin Miniprep Kit (50) from QIAGEN - Sample to Insight. Spin Miniprep Kit (50); CompanyQIAGEN - Sample to Insight; Catalog Number27104.Protocol: Plasmid DNA Purification Using the QIAprep Spin Miniprep Kit . 27104. (250). 27106. QIAprep 2.0 Spin Columns. 50. 250. Buffer P1. 20 ml. 1 x 20 ml, Cat No./ID: 27104. QIAprep Spin Miniprep Kit (50). €97.00. Order Product . Where can I find a protocol for cleanup of already purified plasmid DNA? FAQ ID - Products · Discovery & Translational Research · DNA & RNA Purification · DNA · Cell-Free DNA · DNA Clean Up · Genomic DNA · Microbial DNA · Plasmid DNA.