RNAscope® HiPlex assay requires the reagent kit in conjunction with user specified target probes which contain a mixture of short oligonucleotides designed to bind to a specific target RNA. Each target probe must be assigned to a different probe tail/type (T1-T12). After a series of highly effective and specific signal amplifications, the signal is detected iteratively, where a single RNA Bio-Techne developed the RNAscope HiPlex V2 assay to enable deeper understanding of vital gene expression patterns at a single cell resolution, enabling researchers to generate precise gene expression data from 12 targets in FFPE samples and up to 48 targets in fresh and fixed frozen sample types. HiPlex V2 enables direct visualization of the transcript and eliminates the need for difficult fileslib. We also show how RNAscope WH-FISH can be combined with immunofluorescence (IF) of some epitopes that remain after proteinase digestion and, to some extent, with fluorescent protein markers such as tdTomato. Our WH-FISH technique provides an approach to detect cell-specific quantitative differences in developing and mature adjacent cells, an emerging issue revealed by improved cellular The RNAscope® Multiplex Fluorescent assay is ideal for multiplex ISH to analyze simultaneously mRNA levels of up to three different genes to enable gene exp

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