The pour plate method of counting bacteria is more precise than the streak plate method, but, on the average, it will give a lower count as heat sensitive microorganisms may die when they come contact with hot, molten agar medium. Uses: The pour plate technique can be used to determine the number of microbes/mL in a specimen. Streak Plating 1. Streaking is a technique used to isolate a pure strain from a single species of microorganism, often bacteria. Samples can then be taken from the resulting colonies and a culture can be grown on a new plate so that the organism can be identified, studied, or tested. 2. 2. Streak Plate Procedure: Isolation of Bacterial Colonies Using the Quadrant Method. The streak-plate procedure is designed to isolate pure cultures of bacteria, or colonies, from mixed populations by simple mechanical separation. Single colonies are comprised of millions of cells growing in a cluster on or within an agar plate (Figure 1). A Streaking culture plates in bacteriology by Dr.T.V.Rao MD. What is streaking • The most common method of inoculating an agar plate is streaking. •Streak plates • 1.With this method, a small amount of sample is placed on the side of the agar plate (either with a swab, or as a drop from later in this course. Simpler methods for isolation of a pure culture include: (i) spread plating on solid agar medium with a glass spreader and (ii) streak plating with a loop. The purpose of spread plating and streak plating is to isolate individual bacterial cells (colony-forming units) on a nutrient medium. The disk diffusion method is performed using Mueller-Hinton Agar (MHA), which . is the best medium for routine susceptibility tests because it has good reproducibility, low in sulfonamide, trimethoprim, and tetracycline inhibitors, and gives satisfactory growth of most bacterial pathogens. How to Plate a Specimen for Urine Culture December 2012 Directory of Services - Collection & Handling 4. Inoculate the plates as follows: Void & Cath Cloudy & Foley BA 0.001 BA 0.001 MAC 0.001 MAC 0.001 CNA 0.001 5. Incubate the plates in a 350 C air incubator. 6. Streak Plate Method of Isolation. Purpose The streak plate technique is the most widely used method of obtaining isolated colonies from a mix of cultures.. Principle The streak plate technique is essentially a method to dilute the number of organisms, decreasing the density. This allows for individual colonies to be isolated from other colonies. Method used for inoculating the solid media depends upon the purpose of inoculation- whether to have isolated colonies or to know the bacterial load of the sample (quantitative analysis). For obtaining the isolated colonies streaking method is used, the most common method of inoculating an agar plate is streaking. Fig. 10.6: Streaking method In microbiology, streaking is a technique used to isolate a pure strain from a single species of microorganism, often bacteria. Samples can then be taken from the resulting colonies and a microbiological culture can be grown on a new plate so that the organism can be identified, studied, or tested.The streaking is done using a sterile tool INOCULATION OF AGAR PLATES USING A SWAB A sterile swab must be used, care must be taken not to contaminate by touching other surfaces. Transfer to the growth media as quickly as possible. Depending on your purposes, you can inoculate the swab in a few ways. Described below is the Zigzag method of inoculation. Lab 2b: Introduction to Streaking and Culture Maint