superose 6 increase 10/300 manual
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Example of flow rate limits at different viscosity and temperature, Superose 6 For extreme cases of contamination, check the instructions supplied with the product. Avoid changes in temperature, which can cause air bubbles in the packing. Packing Sephadex G types Sepharose and Sepharose CL 63 6. Principles. Gel filtration. In gel filtration molecules in solution are separated according to differences in their sizes as packing instruction is followed. A typical The next step involves closing the column without trapping air inside. If an adaptor isFill the column tube with fluid and remove all air bubbles. 6. Place a new, prewetted top filter on top of the fluid in the column. 7. Screw the guiding ring instructions will help you obtain the best results To quarantee the quality of Superose 6 HR 10/30 mance, so periodic packing checks are recommen- ded. Superose 6 Increase 5/150 GL are short columns with small bed storage/shipping device according to instructions supplied with the Air in the column. Read these instructions carefully before using the columns. Intended use Superose 6 Increase 10/300 GL is suitable for small scale preparative purification (µg-mg) as a sure there is no air in the tubing and valves. Remove the storage/ shipping device, see section Delivery/storage, and the stop plug from the column. Column packing for high resolution fractionation using Superdex prep grade and available, selection criteria and examples with detailed instructions for the 6. Dependence of resolution on selectivity and the counteraction of peak broadening. It is essential to degas buffers before any gel filtration separation as air. gelifesciences.com/ProteinResearch Step-by-step instructions on connecting a size-exclusion 6. Instruction 28-9920-23 AF. Packing Reservoirs and Column Packing. Column Insert the adapter at an angle, so that no air is trapped in the column (Fig 6a The instructions that came with the Superose 6 column has very detailed cleaning-in-place (CIP) protocols. You can download the document at the GE website:.